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ALL AGAINST AGEING

FUNDAMENTAL CARE
DANIQUE LABORATORIES

What is Stem Cell?
Stem Cell Technology
Stem Cell Benefit
Performance Sources

What is Stem Cell?

Stem Cell is located everywhere in our body and it’s the only cell that can transform into any other cells and rep-product itself, it’s a source cell that have the capacity to differentiate into specialized cells like skin cells, blood cells, nerve cells and many more. That’s the reason why there are many treatment cases by using stem cell, especially to recovery of damages that must be treated in fundamental way.

Do we use Stem Cell? In what way?

What we use is stem cell culture medium, we can get it while stem cell trying to growing by itself in special environment. When stem cell growing, it discharges tons of great nutritious substances and we normally called them as Growth Factors. As for the research, there are more than 50 growth factors inside the substance and we infuse them to our product with high level technology.

Stem Cell Technology

STeMiN™ is CEFO Research Center’s Patent name of adipose-derived stem cell conditioned medium used as cosmetic ingredient. STeMiN™ secured patents for cosmetic effects such as regeneration, whitening, elasticity enhancement, anti-oxidation and anti-inflammation. Safety of STeMiN™ has been proven by animal toxicity tests and human patch tests in accordance to KFDA guidelines.

Powerful Effects

+ Regeneration
+ Anti-Inflammation
+ Moisturizing
+ Anti-oxidation
+ Whitening
+ Anti-wrinkle

Growth Factors

Vascular endothelial growth factor (VEGF)
Transforming growth factor (TGF)-beta
Basic fibroblast growth factor (BFGF)
Hepatocyte growth factor (HGF)
Platelet-derived growth factor (PDGF)
Placenta growth factor (PLGF)
Keratinocyte growth factor (KGF)
Fibroblast growth factor (FGF)

Extracellular Matrix

Type 1 Collagen
Hyaluron Acid

Reference Journal of dermatological science (2007) 48, 15-24 / Journal of dermatological science (2008) 49: 133-142 / Wound rep reg (2007) 15: 540-548 / Brain pathol (2007) 17: 263-275 / Circulation (2004) 109 : 52-58

STeMiN™ 3D Culture System

2D culture method, general cell culture method
3D culture method, cell culture method developed by CEFO Research Center.

2D vs 3D

ROS scavenger (removal of free radical) effect and growth factor secretion were better with 3D than with general cultivation(2D).

ROS Scavenger

Cytokine Secetion

Hyaluronic acid in 3D is also much better than with general cultivation(2D).

Hyaluronic acid

Stem Cell Benefit

Promotes Skin Elasticity

As a result of processing STeMiN on skin cells, the level of mRNA expression of the enzymes involved in collagen synthesis and collagen synthesis promotion was confirmed. “Excellent compared to the control group”

B-actin

Control STeMiN
Col I

Control STeMiN
Col IV

Control STeMiN
Lysyl Hydroxylase (LH)

Control STeMiN

Collagen Type 1 Synthesis

Collagen mRNA Expression

Excellent Whitening Effect

Confirmed the result for decreasing effect of pigmentation by applying SteMiN to melanin pigment.

Melanin Inhibition of SRP1

Melanin Inhibition, According to the STeMiN Concentration

Promotes Skin Regeneration

Growth Promotion of Skin-Derived Cells

Confirmed result : STeMiN applied cells regenerated much better then control

Effect of STeMiN in the skin cells

ROS Scavenger

Removal of Reactive Oxygen Species(ROS)
Checking ROS Level through Skin-derived cells(Keratinocyte and Fibroblsts)
ROS has removed remarkably from all the skin-derived cells when STeMiN applied

STeMiN as a ROS Scavenger in the Keratinocyte

Reactive Oxygen Species concentration

STeMiN as a ROS Scavenger in the Fibroblsts

Reactive Oxygen Species concentration

Anti-inflammation

As a result of inducing an immune response by treating the skin cells with NiSO4 and LPS, which are powerful inflammatory and immune response inducing substances, STeMiN treatment significantly reduces the secretion of cytokines involved in inducing the immune response.

Inflammation response control

Inflammation related cytokine secreation : IL1-α

Inflammation related cytokine secreation : IL1-β

Inflammation related cytokine secreation : IL1-α

Inflammation related cytokine secreation : IL1-β

Patents List : STeMiN™

Composition for whitening containing proteins derived from mesenchymal stromal cells.

KR 10-1716850
(Whitening)
Composition for suppressing immune response containing proteins derived from mesenchymal stem cell.

KR 10-1646236

JP 6273036

PCT/KR2014/001977 (ANTIINFLAMMATION)
Composition for tissue regeneration containing proteins derived from mesenchymal stem cell.

KR 10-1623189 (REGENERATION)
Composition for antioxidation containing proteins derived from mesenchymal stem cell.

KR 10-2014-0038533 (ANTI-OXIDATION)
Composition for promoting the synthesis of extracellular matrix containing proteins derived from mesenchymal stem cell.

KR 10-2014-0038536 (ELASTICITY)

Performance Sources

11 + Patent Ingredient in DANIQUE Excellent for Anti-Ageing.

BioDTox™

is a synergic combination of three plant extracts for detoxification.
Efficiently inhibits the formation of free radicals and protects skin from cell death. It gives not only protection from environmental stresses, but also improve wrinkle formation by boosting collagen synthesis.
TriEx™

Enhancing skin elasticity & giving nutrition TriEx is extracted from Ecklonia, Kelp and seaweed, these are often called Retinol in the ocean since it contains lots of Vitamin A, minerals, Fucoidan* Fucoxanthin.*
*Fucoxanthin gives great vitality to the skin cell *Fucoidan performs as skin lifting, anti-wrinkle, anti-inflammation and anti-tumor
Fuligo™

Superior anti-ageing prescription from the sea Fuligo contains lots of Fucoidan* from natural seaweed from Jeju Island in South Korea. Great performance for improving wrinkles and lifting effects. *Fucoidan performs as skin lifting, anti-wrinkle, anti-inflammation and anti-tumor
PhytoG Stem cell-Edelweiss

Strong Anti-Ageing agent which inhibits the Oxidative stress from free radicals.
Effective for preventing skin damage and even helping regenerate the tissues.
LotuStem™

Effects for increase skin immunity and regenerating. Nature source (Andorographis paniculata) stem cell activator for aged skin sensitive skin and dry skin.
AcePep-6™

Powerful source of Wrinkle care & Anti-Ageing. AcePep-6 is also called Acetyl hexapeptide-8 that has very similar molecular structure with BOTOX®. Alleviate tension level of wrinkle remarkably by restrain muscle activity and decreasing muscle fiber tension.
HerbEx Centella Extract™

Improves cell proliferation and collagen Synthesis at the wound site.
Highly effective in Anti-Irritation, Anti-inflammation.
Epseama™

The first active ingredient in the market that targets a long non-coding RNA to fight the three main cause of ageing. Dramatic Anti-ageing result to over 40’s.
MadeWhite™

Dual function (Whitening+Anti-inflammation) More effectively inhibit post-inflammatory hyper pigmentation.
Clinical Report, Clinical Evaluation.